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KMID : 0525720150200020106
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Journal of Chitin and Chitosan
2015 Volume.20 No. 2 p.106 ~ p.114
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Optimization of High Hydrostatic Pressure Enzymatic Hydrolysis from Flatfish Byproducts using Response Surface Methodology
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Choe In-Hu
Lee Sang-Hoon
Jeon Hyeon-Jin
Kim Yoon-Sook
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Abstract
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High hydrostatic pressure (HHP) enzymatic hydrolysis was applied to the flatfish byproducts in order to develop the functional food materials. Proximate compositions of FFP were determined and its crude protein content, which could be converted to bioactive peptide, was 20.4%. The degree of hydrolysis (DH) of FFP hydrolysates were investigated depending on the process parameters such as pressure, temperature, substrate/enzyme (S/E) ratio, pH and type of enzyme. The results showed that temperature, S/E ratio and pH are major independent parameters to optimize a low-cost commercial process under 100 MPa fixed pressure using Protamex protease. Response surface methodology (RSM) was applied to study the effects of three major HHP enzymatic hydrolysis parameters, and the RSM statistical analysis results showed the hydrolysis parameters for optimal DH; S/E ratio of 50.65% (w/w), pH 6.898 and temperature of 56.97 o C. Under the optimal condition, the DH of FFP was predicted as 32.09%. To enhance the bioavailability, flatfish byproducts hydrolysates were processed through a series of ultrafiltration membrane with molecular weight cut-off (MWCO) 10, 5 and 3 kDa. Four types of permeates including below 3 kDa, 3-5 kDa, 5-10 kDa and over 10 kDa were obtained and a comparative analysis of amino-acid compositions was conducted. These results suggest that HHP enzymatic hydrolysis is an effective processing method for utilization of flatfish byproducts as functional food materials.
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KEYWORD
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High hydrostatic pressure, Enzymatic hydrolysis, Flatfish byproducts, Response surface methodology
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